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Pierce C18 Spin Columns
Pierce C18 Spin Columns. Afterward, desalting columns were packed with c18 beads (0.01 mg/ml in acetone), and cell capture chambers were degassed using pbs solution in order to achieve well dispersed cell flow into the. 2 m iaa in ethanol.prepare immediately prior to use.
50 mm sodium acetate, 150 mm sodium chloride, 10% glycerol (v/v), and degassed ddh 2 o. For peptide cleanup, peptide desalting spin columns (pierce) were used, according to the manufacturer’s guideline. Agilent) with a linear gradient from 0 to 20% acetonitrile over 6 min after an initial hold at 0%.
250 U/Μl In Dh 2 O.
Aliquot in 1.5 ml amber tubes, store at − 20 °c. For peptide cleanup, peptide desalting spin columns (pierce) were used, according to the manufacturer’s guideline. Agilent) with a linear gradient from 0 to 20% acetonitrile over 6 min after an initial hold at 0%.
Afterward, Desalting Columns Were Packed With C18 Beads (0.01 Mg/Ml In Acetone), And Cell Capture Chambers Were Degassed Using Pbs Solution In Order To Achieve Well Dispersed Cell Flow Into The.
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2 M Iaa In Ethanol.prepare Immediately Prior To Use.
Collected peptides from the 10 subcellular fractions were quantified with qubit (manufacturer’s details), and 80 μg of peptides from each fraction was taken and dried before tmt labeling according to the manufacturer’s. 125 u/μl in dh 2 o. 50 mm sodium acetate, 150 mm sodium chloride, 10% glycerol (v/v), and degassed ddh 2 o.
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